Development of UV Spectrophotometric Fingerprint Method for Evaluation of Sitopaladi Churna

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Sitopaladi churna is a most trusted and sold Ayurvedic formulation among the Ayurvedic medicines. Sitopaladi churna contains sugar, Bombusa bombos (Banslochan), Piper longum (Pipali), Elattaria cardamomum (Elaichi), Cinnomomum zeylanicumm (Tvak). Piperine, 1-[5-(1,3-Benzodioxol-5-yl)-1-oxo- 2,4-pentadienyl] piperidine is the alkaloid [1] a biomarker constituent of Piper longum (fruit) responsible for the pungency of piper longum( fruit) .It has also been used in some forms of traditional medicine and as an insecticide. Piperine extract was prepared from the fruits of Piper longum and two lab formulations and three marketed formulations. Piperine was used as a reference standard for analysis of P. longum in lab formulations and marketed formulations. Content of piperine in different batches of Sitopaladi churna (laboratories and marketed batches) was found to be 0.456 ± 0.015%, 0.432 ± 0.022% and 0.188 ± 0.002 %, 0.172 ± 0.001% and 0.165 ± 0.001 % w/w respectively for SL-I, SL-II and SCM- A, SCM-B and SCM-C. A rapid accurate and precise method was developed in the laboratory. The proposed method can be used for uv-spectrophotpmetric fingerprint analysis of piperine in Sitopaladi churna.

Piperine; fingerprint; UV spectrophotometer; Sitopaladi churna