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Nassar O. M, Amin M. A, Hassan M. E, Nasr H. A. Identifying The Natural Antioxidants and Total Phenols of Some Date Varieties in Saudi Arabia.
Manuscript received on : 10 May 2016
Manuscript accepted on : 06 June 2016
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Identifying The Natural Antioxidants and Total Phenols of Some Date Varieties in Saudi Arabia

Olfat M. Nassar1, Mohamed A. Amin2, Mohamed E. Hassan3, 4and Heba Ali Nasr5

1Department of Home Economics (Nutrition), Faculty of Science and Arts, Northern Border University (Rafha), Saudi Arabia. 2Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt. 3Center of Excellence, advanced material & Nanobiotechnology Group, National Research Centre, 12622 Dokki, Giza, Egypt. 4Chemistry of Natural and Microbial Products Department, National Research Centre, 12622 Dokki, Giza, Egypt. 5Department of Biology, Faculty of Science and Arts, Northern Border University (Rafha), Saudi Arabia.

Corresponding Author E-mail: mohassan81@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2108

ABSTRACT: Phoenix dactylifera L. (date palm) is well known for its innumerable health benefits and nutritional values. Therefore, the present work aimed to determine the total phenolic content in five date palm fruit varieties, Barhi, Barni Al Madina, Khesab, Sukkary and  Ajwa, collected from different regions of Saudi Arabia , extracted with aqueous method and to evaluate in vitro their antioxidative properties by DPPH method. Consequently, the total polyphenol contents of these extracts will be measured using Folin Ciocalteu spectrophotometric method. Total phenolic content ranged from 57.8 and 55.2 mg of gallic acid equivalent /30 g fresh wt. and the antioxidant activity was ranging from a low value of 29.2 in Sukkary cultivar to a high value of 31.8 in Barni Al Madina cultivar. Also the best carrier for encapsulation of date Palme extracts is CMC comparing with alginate and carrageenan.

KEYWORDS: Phoenix dactylifera,Encapsulation,total phenolic content,antioxidant activity

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Nassar O. M, Amin M. A, Hassan M. E, Nasr H. A. Identifying The Natural Antioxidants and Total Phenols of Some Date Varieties in Saudi Arabia.

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Nassar O. M, Amin M. A, Hassan M. E, Nasr H. A. Identifying The Natural Antioxidants and Total Phenols of Some Date Varieties in Saudi Arabia. Biosci Biotech Res Asia 2016;13(2). Available from: https://www.biotech-asia.org/?p=12676

Introduction

The role of traditional medicines in the solution of health problems is invaluable on a global level. Medicinal plants continue to provide valuable therapeutic agents, both in modern and in traditional medicine [1].

Date palm is native to North Africa and Persian Gulf regions but its exact origin is uncertain. Top ten producer of Phoenix dactylifera are Iraq, Egypt, Saudi Arabia, Tunisia, Algeria, UAE, Oman, Libya Arab Jamahiriya, Pakistan, Sudan, Europe, and USA [2].

The date palm (Phoenix dactylifera L.) is one of oldest cultivated plants of human kind and used as food for 6000 years [3]. There are more than two hundred varieties [4] of dates available worldwide. It is the main crop in Egypt, Saudi Arabia, and Middle Eastern countries. Each type of dates has shown medicinal value in various type of disease prevention. Dates and their constituents show a role in diseases prevention through anti-oxidant, anti-inflammatory and anti-bacterial activity.

Date palm fruits are one of the most popular fruits packed with an impressive list of essential nutrients, vitamins, and minerals that are required for normal growth, development and overall well-being. They contain health benefiting poly phenolic antioxidants [5].

Dates, fruits of the date palm (Phoenix dactylifera L.) are a main source of staple food in arid and semi-arid regions of North Africa, middle east and South-Asian countries. Dates have always played an important role in the economic and social lives of people of this area. Algeria is ranking the fifth world producer of date palm fruits with production about 710,000 tons occupying an area of 170000 hectares [6]. There are over 58 different varieties of dates in region of Ouargla [7]. Moreover, recent Studies have shown that date fruits are an excellent source of phenolics and therefore possess an extremely high antioxidant capacity. Dates have potent anthocyanins, carotenoids, and phenolics compounds (protocaechuic, p-hydroxy benzoic, vanillic, syringic, caffeic, coumaric, ferulic, hydroxy benzoic, mainly cinnamic acids) and flavonoids (flavones, flavonols and flavanones). As of today, dates also have the unique distinction of being the only food to contain flavonoid sulfates, which like most other fruits, have antioxidant properties [8].

Phoenix dactylifera L. commonly known as date palm is one of the oldest and most popular fruit trees in the hot arid regions of the world, particularly in the Gulf countries of the Middle East [9]. Date fruits are a good source of essential nutrients, including sugars, proteins, fibers, trace elements, etc., and form an important part of the daily diet [10]. They are also popular in other parts of the world due to their delicious taste, nutritional value and health benefits. Although ripe and mature sun dried dates are consumed throughout the year, but during the holy month of Ramadan their consumption increases many folds as most of the Muslims break their fast with dates [11]. It is considered to be an important subsistence crop in dry and semi dry regions of the world due to its socioeconomic and traditional importance. [12, 13]

Based on the traditional Arabic practice and accepted international terminology, dates are classified into five stages viz. Hababouk (first stage which lasts for 4-5 weeks post fertilization), Kimri (green stage, lasts for 9-14 weeks), Khalal (color stage), Rutab (soft ripe stage) and Tamar (full ripe stage) [14]. Dates are available in different shape and size. The ripe dates are; oval-cylindrical in shape, usually 3- 7 cm long, 2-3 cm diameter and bright red to bright yellow in color depending on the variety. Dates contain a seed (pit) which is about 2-2.5 cm long and 6-8 mm thick and is approximately 13-15% of date’s weight [15]. However, fruit quality is influenced by many factors such as size, color, texture, cleanliness, freedom from defects and the effects of decay-causing pathogens.[16,17]

Dry dates contain approximately 70% of carbohydrates in the form of simple sugars that makes them high energy food. In addition to the macromolecules and other essential micronutrients, phytochemicals like flavonoids, carotenoids, phenolic acids, sterols, procyanidins, and anthocyanins are also present in the dates [18]. Because of these antioxidant phytoconstituents, dates are used in traditional system of medicine for the treatment of hypertension, atherosclerosis, microbial infections, constipation, diabetes and cancer [19]. Consumption of dates on a regular basis is even considered to be beneficial in increasing sexual stamina, reducing sterility caused by various sexual disorders, decreasing fatigue and sluggishness in anemic patients [20]. (Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date (Phoenix dactylifera L.) varieties of Oman). Encapsulation is a process in which thin films, generally of polymeric materials are applied to little solid particles, liquid or gases droplets. This method is used to trap active components and release them under controlled conditions. Several materials have been encapsulated in the food industry, among others, aminoacids, vitamins, minerals, antioxidants, colorants, enzymes and sweeteners [21].

The main objective of encapsulation is to protect the core material from adverse environmental conditions, such as undesirable effects of light, moisture, and oxygen, thereby contributing to an increase in the shelf life of the product, and promoting a controlled liberation of the encapsulate ( 22).The encapsulating agents used in this study were carrageenan, carboxymethyl cellulose (CMC) and alginate. They have several polar groups such as –OH and –NH which can act as electron donors [23].

Materials

Sodium alginate was purchased from Sigma-Aldrich, (Germany). Calcium chloride (CaCl2) and potassium chloride KCl were purchased from Park Scientific limited (UK). K– Carrageenan was purchased from Sigma-Aldrich, (Germany). Carboxy Methyl Cellulose (CMC) was purchased from Sigma-Aldrich, (Germany). Ferric chloride (Fe Cl3) was purchased from Sigma-Aldrich, (Germany). Gallic acid, Caffeic acid, p-Coumaric acid, Vanillic acid, Syringic acid (Figure 1), DPPH, Sodium carbonate, Foline Ciocalteu’s reagent and methanol were purchased from SigmaeAldrich, USA. All other chemicals and reagent used were obtained locally and were of high purity.

 Figure (1): phenolic acids Figure 1: phenolic acids

 

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Methods
The date fruits were pitted and sliced to pieces with the approximate dimensions of 1cm × 1cm × 0.3cm. Then the prepared date fruits were used extraction methods.

Sample collection

Five different varieties of P. dactylifera L. namely collected from different places from Saudi Arabia. Palm dates fruits of uniform size, free of damage,insects and fungal infections were selected and used for all experiments. Samples were washed with tap water and the pits were removed by hand for preparation of water extract.

Extraction of date syrup with water

Date pulp (30 gm) putted in an Erlenmeyer flask (250 ml) and 100 ml of water were added and samples were blending using a hand-held blender (Phillips, Holland). The pH was adjusted to 6.0 ± 0.2. Each sample, in triplicate, was placed in water bath at 70°C for 2 h. After heating the slurry was filtered through a cheese cloth with a hand press to remove large impurities and insoluble matters, then the supernatant was decanted and weighed [24].

Preparation of polymer – date syrup beads

Alginate – date syrup beads

Sodium alginate was dissolved in water extract of date to produce polymer solutions with a concentration of 2 % (w/v), the solutions was left standing for 3 hrs. to disengage bubble before use. Fifty milliliters of alginate-date emulsion was then sprayed into a collecting water bath containing calcium chloride solution 2% (w/v)  by using  an Inotech Encapsulator (figure 2)  (Switzerland) with a 450 µm nozzle. The resulting microcapsules were allowed to harden in the CaCl2 solution for 3 hrs.  Alginate-date beads were collected from the cross-linking solutions using a sieve. Finally, the microbeads were rinsed twice with distilled water; tissue paper was used to absorb the surface excessive water onto the wet microcapsules [25].

 Figure (2): Encapsulator for making uniform gel beads. Figure 2: Encapsulator for making uniform gel beads.

 

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K- Carrageenan – date syrup beads

K– Carrageenan was dissolved in water extract of date at 65 C to produce polymer solution with a concentration of 2 % (w/v), the solution was left standing for 3 hrs. to disengage bubble before use. Fifty milliliters of K– Carrageenan-date emulsion was then sprayed into a collecting water bath containing 2% (w/v)  potassium chloride solution by using  an Inotech Encapsulator (figure 2)  (Switzerland) with a 450 µm nozzle. The resulting microcapsules were allowed to harden in KCl solution for 3 hrs.  The beads were collected from the cross-linking solutions using a sieve. Finally, the microbeads were rinsed twice with distilled water; tissue paper was used to absorb the surface excessive water onto the wet microcapsules [26].

CMC – date syrup beads

Carboxy Methyl Cellulose (CMC) was dissolved in water extract to produce polymer solution with a concentration of 2 % (w/v), the solution was left standing for 3 hrs. to disengage bubble before use. Fifty milliliters of CMC-date emulsion was then sprayed into a collecting water bath containing 0.05 M ferric chloride solution by using  an Inotech Encapsulator (figure 2)  (Switzerland) with a 450 µm nozzle. The resulting microcapsules were allowed to harden in FeCl3 solution for 3 hrs.  The CMC beads were collected from the cross-linking solutions using a sieve. Finally, the microbeads were rinsed twice with distilled water; tissue paper was used to absorb the surface excessive water onto the wet microcapsules [27].

Determination of total phenolic content

The total phenolic content was determined according to the Folin-Ciocalteu procedure [28]. Briefly, the extract was transferred into a test tube and the volume adjusted to 3.5 ml with distilled water and oxidized with the addition of 250 µl of Folin-Ciocalteau reagent. After 5 min, the mixture was neutralized with 1.25 ml of 20% aqueous Na2CO3 solution. After 40 min, the absorbance was measured at 725 nm against the solvent blank. The total phenolic content was determined by means of a calibration curve prepared with gallic acid, and expressed as milligrams of gallic acid equivalent (mg GAE) per gm of sample. Additional dilution was done if the absorbance value measured was over the linear range of the standard curve.

Determination of radical DPPH scavenging activity

Free radical scavenging capacity of extracts were determined using the stable 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) according to Hwang and Do Thi (2014) [29]. The final concentration was 200 µM for DPPH• and the final reaction volume was 3.0 mL. The absorbance at 517 nm was measured against a blank of pure methanol at 60 min.

Results

Total phenolic content in five date cultivers

The total content of phenolic compounds in date cultivars is expressed as mg of gallic acid equivalent in 30 g fresh weight and is presented in table 1.

Table 1: Total phenolic content in five date cultivers

Type of variety Total phenolics
(mg of gallic acid equivalent / 30 gm fresh wt.)
A 57.1 ± 0.02
B 57.8 ± 0.03
C 56.9 ± 0.15
D 56.3 ± 0.01
E 55.2  ± 0.02

A: Barhi, B: Barni Al Madina, C: Khesab, D: Sukkary and E: Ajwa.

Total phenol as Caffeic acid equivalent (CAE) is expressed as Mean SD, n ¼ 3; Means within a column with no common letter differ significantly (p < 0.05). Based on the previous results, the Barni Al Madina was the highest variety of the total phenolics.

The antioxidant activity in five date cultivers

The antioxidants activities of five date cultivers were investigated by commonly used DPPH radical scavenging method. The scavenging effect of hydroalcoholic date extracts on the DPPH is expressed as % inhibition. The antioxidant activity equivalent in 30 g fresh weight and is presented in table2.

According to the results recorded in the above table, it was found that, the Barni Al Madina was the highest activity among the tested varieties.

Total phenolic content for immobilized date extract

Total phenolic content of immobilized date extract were investigated according to the Folin-Ciocalteu procedure. And from the results shown in figure (3) we notice that the best carrier for encapsulation of date Palme extract is CMC this is may be due to it has more carboxylic groups, then alginate which possess beside carboxylic groups it has also more hydroxyl groups than carrageenan.

 Figure (3) Total phenolic content for immobilized date extract Figure 3: Total phenolic content for immobilized date extract

 

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The antioxidant activity for immobilized date extract

The antioxidant activities of immobilized date extract were investigated by commonly used DPPH radical scavenging method. The scavenging effect of hydroalcoholic date extracts on the DPPH is expressed as % inhibition. And from the results shown in figure (4) we notice that the best carrier for encapsulation of date Palme extract is CMC this is may be as it has more carboxylic groups, then alginate which possess beside carboxylic groups it has also more hydroxyl groups than carrageenan.

 Figure (4): The antioxidant activity for immobilized date extract Figure 4: The antioxidant activity for immobilized date extract

 

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Discussion

The total phenols assessed using Folin–ciocalteu phenol’s reagent showed that the Barni Al Madina possessed significantly higher amount of phenolics than the other types of cultivers with the values of 57.8 mg of gallic acid equivalent (Table 1). At the cultivar level, total phenolics ranged from 57.8 and 55.2 mg of gallic acid equivalent /30 g fresh wt. Estimates of total phenolics in dates using the colorimetric Folin–Ciocalteu method varies greatly according to variety (11) and phenolic standards used as well as the units used to express the data. Our data are in agreement with previous studies indicating that phenolic content in fruit of date palm decrease as ripening progressed ( 11, 31). Thus, Awad et al.  founded total phenolics concentrations in the range of 50 and 10 mg/100 g fresh weight (FW), in fruits of five date palm cultivars grown in Saudi Arabia (32). In addition, the degree of ripeness considerably affects the concentrations and proportions of the various total phenols in edible plants. In general, it has been observed that phenolic acid concentrations decrease during ripening (33).

The antioxidant activity of fruit extracts was assessed using the DPPH assay. The study revealed that cultivars of the Barni Al Madina exhibited the highest antioxidant activity with an average of 31.8 (%) /30 g FW. Also we found that the antioxidant activity was ranging from a low value of 29.2 in Sukkary cultivar to a high value of 31.8 in Barni Al Madina cultivar (Table 2). The antioxidant activity of dates was reported by several authors (34, 35). Although different assays has been used in the assessment of their antioxidant activity such as DPPH, ferric-reducing antioxidant potential (FRAP), 2,20-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS), they all concluded to the powerful antioxidant activity of date fruit at both ripening stages. Mansouri et al.  using the same assay to estimate the antioxidant activity expressed as the mass ratio (lg sample/lg DPPH) in seven different ripe date palm fruits from Algeria, including Deglet nour, founded an antiradical efficiency that ranged from 0.08 to 0.22.(36).

Table 2: The antioxidant activity in five date cultivars

Type of variety The antioxidant activity /30 gm fresh wt)
A 31.1 ± 0.01
B 31.8 ± 0.02
C 30.9 ± 0.04
D 29.3 ± 0.12
E 29.2 ± 0.02

A: Barhi, B: Barni Al Madina, C: Khesab, D: Sukkary and E: Ajwa.

Conclusion

Date palm (Phoenix dactylifera L) is an important fruit and cash crop in Saudi Arabia, it is socioeconomically and traditionally important for local populations where the culture thrives. Saudi Arabia, being a vast country, has a number of varieties of dates differing. But, unfortunately, it is still to be explored. The results presented in this study showed that the date palm fruit from Saudi Arabia has antioxidant activity and phenolic content. On the basis of our finding, we conclude that date palm fruit constitutes a natural source of potent antioxidants that may prevent many diseases and could potentially be used in food and neutraceutical formulations. In the other hand it can be encapsulated in different types of bio polymeric matrix and from the data obtained we found that CMC is the best one then alginate and carrageenan come last one.

Acknowledgments

The authors wish to acknowledge the approval and the support of this research study by the grant No (8 / 26 / 1436 / 5) from the Deanship of the Scientific Research in Northern Border University (N.B.U.), Arar, KSA.

Reference

  1. Krentz AJ, Bailey CJ. Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs; 2005; 65: 385 – 411.
  2. Lim TK. Edible Medicinal and Non-Medicinal plants (Fruits). New York: Springer. 2012; Pp. 408.
  3. Sulieman A, Elhafise I, Abdelrahim A. Comparative study on five Sudanese date (Phoenix dactylifera L.) fruit cultivars. Food Nut Sci, 2012; 3: 1245-1251.
  4. Amer WM Taxonomic and Documentary Study of Food Plants in Ancient Egypt. Ph.D. Thesis, Cairo University, Giza. 1994.
  5. El Sohaimy SA, Abdelwahab AE, Brennan CS, Aboul-enein AM. Phenolic content, antioxidant and antimicrobial activities of Egyptian Date Palm (Phoenix dactylifera L.) Fruits. Australian Journal of Basic and Applied Sciences, 2015; 9(1):141-147.
  6. FAO. Statistical Databases: www.FAO.org Accessed, 2013; 20.01.2013.
  7. S. Acourene, A. Allam, B. Taleb, M. Tama, Inventory of the different date palm ( Phoenix dactylifera ) cultivars in the regions of Oued-Righ and Oued-Souf (Algeria). Sécheresse,2007; 18:135–142
  8. Zineb Ghiaba, Mohamed Yousfi, Mohamed Hadjadj, Mokhtar Saidi, Messaouda dakmouche. Study of Antioxidant Properties of Five Algerian Date (Phoenix
    dactylifera L)
    Cultivars by Cyclic Voltammetric Technique Int. J. Electrochem. Sci., 2014; 9: 909 – 920
  9. Saleh EA, Tawfik MS, Abu-Tarboush HM. Phenolic contents and antioxidant activity of various date palm (Phoenix dactylifera L.) fruits from Saudi Arabia. Food Nutr Sci 2011; 2: 1134 – 1141.
  10. Benmeddour Z, Mehinagic E, Le Meurlay D, Louaileche H. Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: a comparative study. J Funct Foods 2013; 5: 346 – 354.
  11. Al-Farsi M, Alasalvar C, Morris A, Baron M, Shahidi F. Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. J Agric Food Chem 2005; 53: 7592 – 7599.
  12. Chandrasekaran M, Bahkali AH. Valorization of date palm (Phoenix dactylifera) fruit processing by-products and wastes using bioprocess technology e Review. Saudi J Biological Sci 2013; 20: 105 – 120.
  13. Jain SM, Al-Khayri JM, Johnson DV. Date palm biotechnology, 1st ed. New York: Springer; 2011.
  14. Baliga MS, Baligab BRV, Kandathilc SM, Bhatd HP, Vayalile PK. A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Inter 2011; 44: 1812 – 1822.
  15. Hussein AS, Alhadrami GA, Khalil YH. The use of dates and date pits in broiler starter and finisher diets. Bioresour Technol, 1998; 66: 219 – 223.
  16. Chao CT, Krueger RR. The date palm (Phoenix dactylifera L.): overview of biology, uses, and cultivation. Hort Sci 2007; 42(5): 1077 – 1082.
  17. Ashraf Z, Hamidi-Esfahani Z. Date and date processing: a review. Food Rev Int 2011; 27(2): 101 – 133.
  18. Baliga MS, Baligab BRV, Kandathilc SM, Bhatd HP, Vayalile PK. A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Inter 2011; 44: 1812 – 1822.
  19. Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). J Ethnopharmacol 2007; 110: 105 – 117.
  20. Selvam ABD. Inventory of vegetable crude drug samples housed in botanical survey of India, Howrah. Pharmacogn Rev, 2008; 2: 61 – 94.
  21. Shahidi, F., & Han, X. Encapsulation of food ingredients. Critical Reviews in Food Science and Nutrition, 1993; 33(6), 501–547
  22. Z. Fang, B. Bhandari Encapsulation of polyphenols – a review. Trends in Food Science & Technology, 2010; 21: 510 – 523).
  23. King, A. H. Brown Seaweed Extracts (Alginates). In M. Glicksman (Ed.). Food Hydrocolloids, 1983; 3: pp. 115. Boca Raton, FL: CRC Press, Chapter 3).
  24. El-Sharnouby GA, Aleid SM, Al-Otaibi MM. Liquid Sugar Extraction from Date Palm (Phoenix dactylifera L.) Fruits. J Food Process Technol, 2014; 5: 402. doi:10.4172/2157-7110.1000402)
  25. Ghada E.A. Awad, Abeer A. Abd El Aty, Abeer N. Shehata, Mohamed E. Hassan, Magdy M. Elnashar, Covalent Immobilization of Microbial Naringinase Using Novel Thermally Stable Biopolymer for Hydrolysis of Naringin.. 3 Biotech, 2016; 6:14
  26. Magdy M. M. Elnashar and Mohamed E. Hassan, “Novel Epoxy Activated Hydrogels for Solving Lactose Intolerance,” BioMed Research International, vol. 2014, Article ID 817985, 9 pages, 2014.
  27. Amal M. Hashem, Amira A. Gamal, Mohamed E. Hassan, Naziha M. Hassanein, Mona A. Esawy, Covalent immobilization of Enterococcus faecalis Esawy dextransucrase and dextran synthesis. International Journal of Biological Macromolecules, 2016; 82: 905–912
  28. Zilic S., Serpen A., Akıllıoglu G., Jankovic M. and Gökmen V. Distributions of phenolic compounds, yellow pigments and oxidative enzymes in wheat grains and their relation to antioxidant capacity of bran and debranned flour. Journal of Cereal Science, 2012;56: 652-658.
  29. Hwang, E. S. and Do Thi, N. Effects of Extraction and Processing Methods on Antioxidant Compound Contents and Radical Scavenging Activities of Laver (Porphyra tenera). Prev. Nutr. Food Sci., 2014; 19:40-48.
  30. Eid, M. N. S., B. Al-Awadi, D. Vauzour, M. J. Oruna-Concha, and J. P. E. pencer. Effect of cultivar type and ripening on the polyphenol content of date palm fruit. J. Agric. Food Chem., 2013;61: 2453–2460.
  31. Hammouda, H., J. K. Ch erif, M. Trabelsi-Ayadi, A. Baron, and S. Guyot. Detailed polyphenol and tannin composition and its variability in Tunisian dates (Phoenix dactylifera L.) at different maturity stages. J. Agric. Food Chem. ,2013;61:3252–3263.
  32. Awad, M. A., A. D. Al-Qurashia, and S. A. Mohamed. Antioxidant capacity, antioxidant compounds and antioxidant enzyme activities in five date cultivars during development and ripening. Sci. Hortic., 2011; 129: 688–693.
  33. Manach, C., A. Scalbert, C. Morand, C. R em esy, and L. Jim enez.. Polyphenols: food sources and bioavailability. Am. J. Clin. Nutr., 2004; 79: 727–747.
  34. Allaith, A. A. Antioxidant activity of Bahraini date palm (Phoenix dactylifera L.) fruit of various cultivars. Int. J. Food Sci. Technol., 2008; 43: 1033–1040.
  35. Chaira, N., A. Mrabet, and A. Ferchichi. Evaluation of antioxidant activity, phenolics, sugar and mineral contents in date palm fruits. J. Food Biochem., 2009; 33: 390–403.
  36. Mansouri, A., G. Embarek, E. Kokkalou, and P. Kefalas. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera L.). Food Chem., 2005; 89: 411– 420.
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