Feasibility Decrease Use of Fertilizer on Greenhouse Cucumber Production in Soilless Culture

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The recombinant plasmid, pSNC3301 carrying the coat protein gene of cucumber mosaic virus (CpCMV) was used in the Agrobacterium-mediated transformation of chili (Capsicum annuum L.). The transformation protocol required 2 days co-cultivation, 2 days pre-selection in non-BASTATM herbicide containing medium, 3-4 weeks selection in BASTATM herbicide containing medium, 20-25 weeks for culturing of adventitious shoot in regeneration medium and finally 1 week in rooting medium. PCR analysis detected the presence of CpCMV gene with transformation efficiency of 1.5% (7/467) for hypocotyl and 0.7% (4/536) for shoot tip. Histochemical GUS assay of hypocotyls, showed only nine from 22 PCR positive transformants showed GUS expression. For shoot tip, three of 13 PCR-positive transformants expressed GUS. The transformation efficiency of coat protein using pSNC3301 was 1.5% (7/467) for hypocotyls and 0.7% (4/536) for shoot tip.re

Capsicum annuum; shoot tip; hypocotyls; transformation; Agrobacterium Coat protein; CMV