In Vitro Clonal Propogation of Capsicum Annum L. Var. (Arka Lohit And Arka Suphal) for Fruit Borer Disease Resistance


R. Bargavi and S. Elumalai
Plant Biology and Biotechnology Department, Presidency College, (Autonomous) Chennai - 600 005 (India).

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ABSTRACT:

An efficient protocol of in vitro clonal propagation has been developed from epicotyls, hypocotyls, cotyledons, leaf disc of capsicum annum. L.var. (Arkalohit and Arka suphal).Different types of explants and developmental stages sub cultured in different media combinations were studied. Explants cultured on murashige and Skooge (MS) medium supplemented with silver nitrate (AgNO3), Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxy acetic acid ( 2,4-D) combination. Shoot regeneration recorded best with 4mg/l AgNO3 and 5mg/l BAP and 2mg/l 2, 4-D combination. The best rooting media was recorded as MS media supplemented with 1.0mg/l NAA and 5.0mg/l IBA.

KEYWORDS:

Capsicum; Clonal propogation; Explants; Epicotyls; Hypocotyls

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Article Publishing History
Received on: December 22, 2009
Accepted on: January 02, 2010


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