In situ absorption study of picroside-i and kutkoside (active principles of picroliv) using lc-ms-ms

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In situ absorption study was carried out to predict the intestinal absorbability of picroliv, in terms of its active moieties (Picroside I and kutkoside). Picroside I and kutkoside were found to be absorbed slowly through the intestine, which may be due to the saturation of the receptors responsible for the absorption by the other constituents present in the picroliv preparation.A rapid, sensitive and selective LC-MS-MS method for the simultaneous quantitation of picroside-I and kutkoside (active constituents of herbal hepatoprotectant picroliv) was revalidated. Analysis was performed on Spheri-5, RP-18 column (10 μm, 100 x 4.6 mm i.d.) coupled with guard column using acetonitrile: triple distilled water (50:50, %v/v) as mobile phase at a flow rate of 1 ml/min. The quantitation was carried out using an API-4000 LC-MS-MS with negative electro spray ionization in multiple reaction monitoring (MRM) mode. The

LC-MS-MS; hepatoprotectant; Picroside-I; Kutkoside; MRM