Argentated silica gel chromatography for separation of g-linolenic acid from microalgae

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A low expense process is developed for recovery of g-linolenic acid from Spirulina platensis biomass. Simultaneous extraction and direct transmethylation of fatty acids from freeze dried biomass and subsequent purification using silver silica gel column chromatography resulted in 95.43% pure g-linolenic acid. The percent purity of g-linolenic acid in the fractions varied from 87.18 -95 %. A recovery of 74.05% g- linolenic acid was achieved using silver silica gel chromatography.

g-linolenic acid; silver silica gel column; chromatography; methyl ester