Invitro culture of mesenchymal stem cells from ovine amniotic fluid cells

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Fetal tissue engineering has emerged as a promising concept in surgical reconstitution of certain birth defects. Utilizing minimally invasive techniques, fetal tissue has been harvested, cultured and manipulated invitro and the resulting autologous cellular bioprosthesis is used for implantation shortly after birth. Thus far, this concept has been used sucessfully in animal experiments for the reconstruction of bladder, skin and diaphragmatic deformities. These preliminary reports are promising and harvesting fetal tissue and its culture could be a best source of stem cells with remarkable plasticity (Temple,2001).

Ovine amniotic; mesenchymal stem cells; In vitro culture