Effect of some inhibitors on hyaluronidase production by Streptococcus mitis MTCC *2695 and Streptococcus equi SED 9 species

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The inhibitory effects of some metal ions, some synthesized compounds and their derivatives with 2-aryl acenaphthimidazoles and their N-Mannich bases on optimum production of hyaluronidase was evaluated employing Streptococcus mitis MTCC *2695 and Streptococcus equi SED 9 by submerged fermentation. The maximum enzymatic activity was obtained with an initial pH (6 and 5.5), incubation temperature 37°, incubation time for 48 h and inoculum level (6 and 10 %) with inoculum age 24 h by Streptococcus mitis and Streptococcus equi SED-9 respectively. The results indicated that metals like Fe3+, Zn2+ Mn2+, Ag+, Ni2+, Sr2+ and Cu2+ acted as strong inhibitors of the enzyme. N-Mannich bases of 2-aryl acenaphthimidazoles exhibited promising inhibitory activity.

Hyaluronidase production; Streptococcus sp.