Effect of herbicides on mitosis of Hibiscus cannabinus Linn.

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The decreases in the mitotic index were observed after treatment of 2,4-D, Oxyfluorfen and Glyphosate.The mitotic index decreased from 8.45 to 3.56 at 1000 to 5000 ppm of Hibiscus cannabinus Linn, 8.26 to 2.84 at 1000 to 8000 ppm of Oxyfluorfen and 9.44 to 4.62 at 5000 to 25000 ppm of Glyphosate. The percentage of abnormalities in root tips cells increased gradually with increase in concentrations of herbicides. The percentage of abnormalities in root tips cells were 0.33 to 3.11 at 2000 to 5000 ppm of 2,4-D, 1.07 to 2.92 at 2000 to 8000 ppm of Oxyfluorfen and 0.62 to 2.54 at 15000 to 25000 ppm of Glyphosate. The abnormalities like binucleolate cells and clumping of chromosomes were observed.

Mitosis; Hibiscus cannabinus; 2, 4-D; Oxyfluorfen; Glyphosate