Callus Formation and Plant induction from axillary buds of Stevia rebaudiana Bertoni (Honey-leaf)

---

Download this article as: 

ABSTRACT:

In vitro regeneration of stevia rebaudiana Betroni. (Honey-leaf) was initiated from axillary buds onto Ms Medium9 , supplemented with different concentration of BAP and KN in higly aseptic conditions. Shoot differentiated best from axillary shoot base callus on MS medium containing BAP (1Mgl-1) where maximum bud break (80%), number of leaves 5-6 and shoot length 2-3 cm. Were shown and in comparision with medium containing KN (1.5 mgl-1) maximum bud break (60%), number of leaves 3-4 and shoot length 1-2 cm were observed. Regenerated shoots were sub cultured for roots formation.

KEYWORDS:

Stevia rebaudiana; BAP-6- benzylaminopurine; KN-6- furfurglaminopurine