Recombinant Colicin producer Lactobacillus acidophilus and its Inhibitory Effect Against UPEC

DOI : http://dx.doi.org/10.13005/bbra/1604

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Lactobacillus species are a predominant member of vaginal microflora and play a crucial role in prevention of a number of urogenital diseases. To increase antimicrobial potential of vaginal lactobacilli, Colicin E2 possessing specific activity against uropathogenic Escherichia (E.) coli was over-expressed in vaginal probiotic lactobacillus(L.) acidophilus. Recombinant L. acidophilus expressing Colicin E2 showed much higher inhibitory activity against uropathogenic E. coli than wild type in vitro. Efficacy of probiotic L. acidophilus expressing colicin E2 protein required for further in vivo evaluation. The present study in consistent with similar studies demonstrated that the production of colicin by vaginal probiotics may increase the preventive effects of lactobacilli against urinary tract infections.

Escherichia coli; Lactobacillus acidophilus; urinary tract infections