Comparitive Study on the Effect of Serum and Plasma on Glucose and Cholesterol Estimation

A. Julius; Rajalakshmi Allen; K. Pramila; A. Venkatesan; Bhuminathan Swamikannu

Article | Open Access

Comparitive Study on the Effect of Serum and Plasma on Glucose and Cholesterol Estimation

A. Julius¹, Rajalakshmi Allen², K. Pramila³, A. Venkatesan⁴ and Bhuminathan Swamikannu⁵

¹Department of Biochemistry, Shree Balaji Dental College and Hospital, Bharath University, Narayanapuram, Pallikaranai, Chennai- 600 100, India. ²Research and Development Department, Bharathiar University, Coimbatore, India. ³Department of Biochemistry, Prince Sri Venkateshwara Arts and Science College, Venkateshwara Nagar, Gowrivakkam, Chennai - 600073, India. ⁴Eshwar medical Foundation, Kolathur, Chennai, India. ⁵Department of Prosthodontics, Shree Balaji Dental College and Hospital, Bharath University, Narayanapuram, Pallikaranai, Chennai- 600 100, India.

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ABSTRACT:

Objective is to study if there is any clinically signiûcant difference between EDTA plasma and serum for glucose and cholesterol estimation. A general problem faced by the clinical laboratories is the integrity of uncentrifuged specimens for chemical analyses. Because prolonged contact of plasma or serum with cell is a common cause of spurious test results, plasma and serum should ideally be separated from cells as quickly as possible to prevent ongoing metabolism of cellular constituents as well as active and passive movement of analytes between the plasma or serum and cellular compartments. (Goodman JR, Vincent J 1954).

KEYWORDS:

Serum glucose; Plasma glucose; Serum cholesterol; Plasma cholesterol; EDTA plasma

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