In vitro Shoot Multiplication in Physalis minima var. Indica L

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DOI : http://dx.doi.org/10.13005/bbra/1137

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ABSTRACT:

Apical shoot buds were taken from in vitro germinated seedlings of Physalis minima. var. indica L. These were placed aseptically on MS (Murashige and Skoog,1962),B5 (Gamborg,1968)basal medium and supplemented with auxins and cytokinins alone. Maximum healthy shoot buds(100 percent) were obtained on MS medium supplemented with 0.5 mgl-1BAP (6- benzyl amino purine) but length of shoot buds was more on (1.0,2.0 mgl-1) Kinetin. Multiplied shoots were transferred for rooting on MS and B5 basal medium and supplemented with auxins (IAA or IBA). Rooting was achieved in 100 percent explants on MS medium supplemented with all the levels of IAA and IBA.Higher root length (11.5+0.50cm) was observed in shoots incubated on 0.5mgl-1 IAA.

KEYWORDS:

Physalis minima; Apical shoot buds; Growth regulators