Enhanced beta-glucosidase production by mutagenesis in Penicillium purpurogenum


A. B. Dhake and M. B. Patil*
University Department of Biochemistry, L.I.T. Premises, Nagpur University, Nagpur - 440 033 (India)

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ABSTRACT:

P. purpurogenum isolated from decaying wood sample in our laboratory produces comparable amount of beta-glucosidase. The intracellular and extracellular enzymes reached to the peak after 4 and 8 days respectively. Germinating spores of this strain were subjected to mutagenesis with U.V and NTG (3-nitro, 5 methyl guanidine). After mutagenesis, both the treatments have shown induction in beta-glucosidase production to significant level.

KEYWORDS:

Beta-glucosidase; mutagenesis; Penicillium purpurogenum

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