Volume 12, number 3
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Optimization and Characterization of Poly[R]Hydroxyalkanoates of Pseudomonas Aeruginosa

Antony. V. Samrot, M Bhakyalakshmi, K. L Venkatraman, K Sahiti, Sheryl-Ann Philip, T Jahnavi and P. Senthilkumar

Department of Biotechnology, Sathyabama University Sholinganallur, Chennai-600119

DOI : http://dx.doi.org/10.13005/bbra/1883

ABSTRACT: Polynucleotides, polypeptides, polysaccharides are the polymers seen in living organisms. Many biopolymers are important reserve compounds that are stored in the cytoplasm as insoluble inclusions. Likewise Polyhydroxyalkanoates are natural, renewable bio poly esters accumulated in bacteria in the presence of limited nitrogen sources and higher concentration of carbon source. In this study, PHA accumulation pattern of Pseudomonas aeruginosa was studied. The PHA was optimized for various carbon sources, incubation time, pH and temperature. Analytical techniques like FTIR and GC-MS were done to determine the types of monomer present in the biopolyester.

KEYWORDS: PHA – Polyhydroxyalkanoates; Pseudomonas aeruginosa; GC-MS; FTIR

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Samrot A. V, Bhakyalakshmi M, Venkatraman K. L, Sahiti K, Philip S. A, Jahnavi T, Senthilkumar P. Optimization and Characterization of Poly[R]Hydroxyalkanoates of Pseudomonas Aeruginosa. Biosci Biotechnol Res Asia 2015;12(3)

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Mardanbeigi D, Ebrahimi M, Bozorgmehr M. R. Preconcentration and Determination of Organophosphorus Pesticides in Water Samples by Carbon Nanotube Reinforced Hollow Fiber Solid/Liquid Phase Microextraction and Gas Chromatography-Flame Ionization Detector. Biosci Biotechnol Res Asia 2015;12(3). Available from: http://www.biotech-asia.org/?p=2123

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