eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 9 2 http://dx.doi.org/10.13005/bbra/1053 10043 article Strong Inhibition of Jack Bean Urease by Chromium (III) G. Rezaei Behbehani 1 L. Barzegar 2 M. Mohebbian 3 Department of Chemistry, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran. Department of Chemistry, Payame Noor University (PNU), Abhar, Iran. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Department of Biological Sciences, Tarbiat Moallem University, Tehran, Iran.

Urease activity is often used for characterization of microbial viability in soil. The aim of the investigations was to measure the influence of chromium (III) on urease activity. Urease activity in pure solution was so sensitive for Cr3+, which caused inhibition of urease activity significantly. In this paper complexation reaction between Cr3+ and Jack bean urease is examined using isothermal titration calorimetry (ITC). It was found that chromium ions acted as a noncooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for Cr3+ ions. The association equilibrium constant is 6.79-106 L-1.mol, indicating the strong interaction of Cr3+ ion with JBU. The molar enthalpy of binding is DH = 15.10 kJmol-1.

 https://www.biotech-asia.org/vol9no2/strong-inhibition-of-jack-bean-urease-by-chromium-iii/ Isothermal Titration Calorimetry Jack bean urease Cr3+ ion binding parameters.