Present study designed to evaluate the effect of Bryophyllum pinnatum Lam. on n-diethylnitrosamine (DENA) induced hepatic injury in rats. The aerial part of B. pinnatum aqueous and ethanolic extract was prepared in the doses of 250mg/kg and 500mg/kg. Hepatic injury was induced by DENA. Acute toxicity was also carried out. Treatment with different doses of ethanolic extract of B. Pinnatum (250 mg/kg, p.o.) not significantly able to treat the liver injury induced by DENA, but 500 mg/kg dose of ethanolic extract of B. Pinnatum slightly protect the liver. Treatment with different doses of aqueous extract of B. Pinnatum (250 and 500 mg/kg, p.o.) significantly (p*<0.05; p**<0.01 and p***<0.001) treat the liver injury induced by DENA. It may be inferred from the present study that the hepatoprotective activities of the aqueous extract of B. Pinnatum leaves in DENA-induced hepatotoxicity may involve its antioxidant or oxidative free radical scavenging activities by alleviating lipid peroxidation through scavenging of free radicals, or by enhancing the activity of antioxidants.