eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 9 2 http://dx.doi.org/10.13005/bbra/1034 9932 article Characterization of Immobilized L-Asparaginase Produced from Pseudomonas aeruginosa 50071 by Solid State Fermentation Mohsen M. S. Asker 1 Sahera F. Mohamed 1.2 Osama H. El-sayed 2 Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt. Chemistry Department, College of Science, Jazan University, Jazan, KSA. The natural polymer sodium alginate is a macropolysaccharide Produced from brown algae has many applications. Anti-leukemia enzyme L-asparaginase (L-ASNase) produced from Pseudomonas aeruginosa 50071 by solid state fermentation was covalently conjugated on sodium alginate. SDS-PAGE showed that the purified L-ASNase was homogeneous with molecular weight of ~ 113 KDa. The immobilized enzyme on 3% sodium alginate showed specific activity of 62 U/mg protein. The immobilized L-ASNase maintained over 90 % of the original activity of free enzyme. Also it showed a slightly thermal stability where the activation energy of denaturation were 28 and 35 Kcal mol-1 for both native and immobilized enzyme, respectively. The Km of sodium alginate immobilized enzyme was about 2 times lower than that of the free enzyme. https://www.biotech-asia.org/vol9no2/characterization-of-immobilized-l-asparaginase-produced-from-pseudomonas-aeruginosa-50071-by-solid-state-fermentation/

Immobilization; L-asparaginase; pseudomonas aeruginosa; solid state fermentation.