eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 9 1 405 410 9721 article M. N. Abubacker 1 A. Ayesha 2 Department of Biotechnology, National College, Tiruchirappali - 620 001, India. Department of Civil Engineering, Kongu Engg College, Perundurai, Erode - 638 052, India. Biodiesel fuel (BDF) produced by alcoholysis of vegetable oils or fats is viewed as a promising renewable fuel source. Diminishing petroleum reserves and increasing environmental regulations have made the search for renewable fuel. Biodiesel is non-toxic and biodegradable, produced from renewable sources and contributes a minimal amount of net green-house gases, such as CO2, SO2 and NO emissions to the atmosphere. The main objective of the present study is to produce biodiesel from vegetable oil and to use micro-emulsions with solvents ethanol and methanol following acid, alkali and fungal enzyme catalysis methods. The best suited method of biodiesel production was ethanolic and alkali mediated trans-esterification process rather than methanolic and acidic trans-esterification. The maximum yield of biodiesel was obtained from Rhizopus oryzae lipase enzyme, ethanolic and alkali mediated trans-esterification followed by Aspergillus niger, Polyporus squamosus and Agaricus campestris. https://www.biotech-asia.org/vol9no1/efficacy-of-fungal-enzyme-in-biodiesel-production-from-vegetable-oil/ Biodiesel; trans-esterification; Fungal enzyme; Lipase