eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 9 1 205 210 9542 article Shahriar Alipour 1 Homayoun Khazali 1 Amir Jalili 2 Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran. Department of Biology, Islamic Azad University, Urmia branch, Urmia, Iran. This study was established for determining the effects of ghrelin in the control of LH (luteinizing hormone) and T (testestrone) hormones secretion in Zell Rams fed by two different levels of their daily food requirements. Ten mature and ten immature rams were selected randomly and divided into two groups (n=5). Animals in 4 groups were fed either 100% or a 50% energy content diaet, for two weeks. Then all rams received jugular vein injection of 5 µg ghrelin/KgBW. Blood samples were withdrawn by jugular venipuncture at 30-minute intervals, 3 h before and 3 h after ghrelin injection. For determining the concentration of LH and T hormones, plasma was isolated and subjected to radioimmunoassay. The results showed that ghrelin injection in both mature and immature rams who were subjected to 50% energy content diaet, significantly reduced T hormone level. Furthermore ghrelin injection in both groups of mature rams significantly reduced LH hormone level. It seems that ghrelin have inhibitory effects on reproduction of Zell Rams by reducing sexual hormones level. https://www.biotech-asia.org/vol9no1/effects-of-ghrelin-on-lh-and-t-hormones-secretion-in-mature-and-immature-zell-rams-fed-by-two-different-levels-of-their-daily-food-requirements/ Ghrelin; Ram; reproduction; LH and T hormones