eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 9 1 423 426 9736 article M. Kannahi 1 K. Rubini 1 PG and Research Department Of Microbiology, Sengamala Thayaar Educational Trust Women's College, Mannargudi- 614 001. India. The soil samples were collected from decomposed polythene bag disposal area. The collected samples were serially diluted and plate . The isolate fungal strains were identified based on their cultural morphological study. The fungal species was isolated and idendified namely Aspergillus oryzae. A. oryzae was subjected to polythene bag degradation based on the resistance capability. Various thickness of polythene bag were prepared. Polythene bag degradation abilities were observed by A. oryzae in soil and heavy metal analysis. In case if A .oryzae shows any loss of thickness and color, it was recorded after one month of incubation period. When size of the polythene bag was 0.5 to 5 mm it shows that fungi can be used in both natural and artificial conditions for the purpose of degradation of polymers and the microbes. Our study was mainly focus on microbes cause greatest degradation of polythene bag. Hence, the further attention is required from microbiologists for commercial degradation and eco-friendly polyethylene with Aspergillus oryzae. In case of A. oryzae loss of thickness was recorded after one month of incubation period when the size of 0.5 to 5mm. The highest degradation of polythene? bag was noted in Pit-3(1.8mm). That indicate the A.oryzae moderately degrade the polythene .? https://www.biotech-asia.org/vol9no1/biodegradation-of-polythene-bag-by-aspergillus-oryzae/ Biodegradation; Polythene bags; Aspergillus oryzae