eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 8 2 729 734 9548 article D. Sengupta 1 S. Ganguly 1 A.K. Banik 1 Department of Chemical Engineering Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, 92, A. P. C. Road, Kolkata - 700 009 (India). Thiobacillus ferrooxidans treated with ethylmethane sulfonate and UV rays was incubated with coal sample taken from Assam, India for its desulfurization capacity. 2% glucose and 0.7% NH4Cl served as the best carbon and nitrogen sources respectively. Optimum concentration of both MgCl2 and KH2PO4 in fermentation medium was 0.025% supplementation of medium with 10 µg/ml Fe2(SO4)3.H2O had marked positive effect on desulfurization. Optimization of chemical nutrients resulted in a significant (p<0.05) impact on desulfurization (66.1%) by the mutant strain Thiobacillus ferrooxidans X200 as against parent strain (55.1%). https://www.biotech-asia.org/vol8no2/stimulatory-effect-of-chemical-nutrients-on-desulfurization-of-indial-coal-by-a-mutant-thiobacillus-ferrooxidans-x200/ Thiobacillus ferrooxidans; Desulfurization; Optimization; Chemical nutrients