eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 8 2 807 811 9660 article P.A. Mary Helen 1 Deepi 1 S. Jaya Sree 1 A.M. Nizzy 1 S.M. Madoen Abisha 1 Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629 153 (India). Random amplified polymorphic DNA (RAPD) analysis was performed on 7 bacterial isolates isolated from leaf litter from canal Kollemcode. The isolates produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Five primers such as OPA-06, OPA-07, OPA-17, OPAD-02, OPAD-07 were used for the RAPD analysis. DNA banding patterns generated by RAPD were scored for the presence (1) or for absence (0) of each amplified band. For genetic distance analysis, using NTSYS software Cluster analysis was based on similarity matrices using the unweighted pair group method analysis (UPGMA) program in the software package. The Jaccard coefficient was used for dendrogram construction. A dendrogram based on these results showed a high level of genetic similarity between different bacterial isolates, and genetic differences were expressed in clusters. The genetic difference between the overall population was low and showed similarity with each population. https://www.biotech-asia.org/vol8no2/rapd-analysis-of-leaf-litter-associated-bacterial-population-from-canal-kollemcode/ RAPD analysis; Dendrogram; Cluster analysis; Genetic distance; Similarity matrix