eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 8 2 841 844 9829 article Ajay Pratap Singh 1 K. D. Sexena 1 Department of Chemistry, Bareilly College, Bareilly - 243 001 (India) A Lectin was isolated from the seeds of Momardica charantia L.var. Jhalri (MCJ) using ammonium sulphate fractionation at pH 6.8 and 4°C. The purification of isolated lectin was done by ion exchange chromatography using DEAE-Cellulose with discontinuous gradient of acetate buffers. Purity and homogeneity of MCJ lectin was confirmed by 10% SDS-PAGE at pH 8.6. The same technique revealed its apparent molecular weight of 128kD indicating it to be a high mol. wt. lectin among the members of Cucurbitaceae family. MCJ lectin showed potent heamagglutination activity for O+ erythrocytes. The total lectin isolated was 118.80 mg per 100 g seeds. Climatic and ecological factors under which the plant grows up were assumed to be responsible for high yield of MCJ lectin. https://www.biotech-asia.org/vol8no2/lectin-from-the-seeds-of-wild-bitter-gourd-momardica-charantia-l-var-jhalri-isolation-and-partial-charactrization/ Lectin; SDS-PAGE; Haemagglutination; Cucurbitaceae