eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 8 2 837 840 9824 article Girraj Singh Meena 1 Department of Botany, Government P. G. College, Dholpur (India). The paper reports the results of application of 5kr, 10kr and 15kr of gamma rays and/ or 0.02% of Nitroso Methyl Urea ( NMU ) on Khesari ( Lathyrus sativus ) var. P-24. Germination, seedling growth and chlorophyll content were adversely affected in all the treatments. Simultaneous treatment with two mutagens was more effective than the single treatment with either mutagen. Results indicated variation for seed germination, seedling growth and chlorophyll content. The seeds treated with 5kr + NMU and 10kr + NMU showed minimum germination. Minimum seedling height was observed in seeds treated with 15kr + NMU (50 mm) on the 15th day after sowing. A little variation in chlorophyll content was also observed. Certain morphological variations were induced in all the treatments. https://www.biotech-asia.org/vol8no2/effects-of-physical-and-chemical-mutagens-on-seed-germination-and-seedling-growth-in-khesari-lathyrus-sativus-var-p-24/ Lathyrus sativus; Gamma rays; NMU; Induced mutation