eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-05 8 2 505 508 9309 article V.C. Khilare 1 S.S. Chavan 1 Botany Research Centre, Vasantrao Naik Mahavidyalaya, CIDCO, Aurangabad - 431 003 (India). Fungicide carbendazim is recommended to manage anthracnose of grapes in orchards which is important disease in India. The wild sensitive isolate GA-1 was studied both in vitro and in vivo on grapes. Culturing wild type isolate continuously for five successive passages on carbendazim individually increased resistance significantly. However, reduced resistance was observed when pathogen was cultured alternately or in mixture with different fungicides of amide and conazole groups. Similar type of results was obtained on the grape berries. Use of difenoconazole and myclobutanil alternately and difenoconazole, myclobutanil and propiconazole in mixture appeared to be most useful to break the development of carbendazim resistance in pathogen. https://www.biotech-asia.org/vol8no2/effect-of-passage-on-the-development-of-carbendazim-resistance-in-gloeosporium-ampelophagum-causing-anthracnose-of-grapes/ Fungicide resistance; Carbendazim; Anthracnose of grapes; Gloeosporium ampelophagum