eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-05 8 1 119 123 9270 article Razzaghi Abolghasem 1 Shahsavar Alireza 1 Pourvaghar Mohammad Javad 2 Physical Education Department, Payame Noor University, 19395-4697 Tehran (Iran). Physical Education Department, University of Kashan, Kashan (Iran). The purpose of this investigation is to study the effect of rest duration in interval training and consumption of vitamin C supplement on serum lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) enzyme concentration. To this end, three groups were selected. The first group had one minute rest between two sets of 400 meters run, the second group had three minutes rest between two sets of 400 meters run and the final group had three minutes rest between two sets of 400 meters run with consumption of vitamin C supplement. The data of the research were analyzed using spss10 software. To test research hypothesis, descriptive statistics with 0.05 significant level and deductive statistics were used. Statistic tests applied were T-test and ANOVA. Each group consisted of ten participants. Results of the investigation indicate that serum CPK and LDH enzymes increasing significantly due to physical activity. Also rest duration did not have significant effect on concentration of serum CPK. The result also showed that the group with more rest time had lower concentration of these enzymes. Vitamin C consumption caused a significant decrease in serum CPK level. https://www.biotech-asia.org/vol8no1/the-effect-of-rest-duration-and-vitamin-c-consumption-on-serum-lactate-dehydrogenase-and-creatine-phosphokinase-concentration-of-men-athletes-during-interval-trainings/ Intermittent Training; Exercise intensity; lactate dehydrogenase; creatine phosphokinase