eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-05 8 1 225 230 9331 article Kannahi 1 K. Ranjani 1 PG & Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tiruvarur(dt), - 614 001 (India). Lipases are the enzymes capable of catalyzing the hydrolysis and synthesis of esters formed from glycerol and long chain fatty acids. The production of lipase from Bacillus subtilis and Aspergillus flavus. After the isolation, lipase producing colonies were screened using tributyrin agar plates, the lipase producing cultures were obtained and maintained as stock cultures. The effect of different pH, temperature, incubation time and substrate concentration on the production of lipase was determined by the organisms on the three different substrates such as olive oil, coconut oil and sunflower oil. Bacillus subtilis produced the maximum amount of lipase in all physical parameters such as pH, temperature, incubation time and substrate concentration when compared to Aspergillus flavus. https://www.biotech-asia.org/vol8no1/lipolytic-potentialities-of-microbes-from-oil-mill-effluent/ Lipase;Tributyrin agar medium; Bacillus subtilis; Aspergillus flavus