eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 7 2 879 888 9396 article B. Manuel Choragudi 1 B. Ranjani Choragudi 1 S. Felice Choragudi 2 V. Saradhi Settaluri 2 Sujay Bio-Tech Pvt. Ltd., Plot No.19A, Phase - III, 3rd Road, Jawahar Autonagar, Vijayawada (India). Koneru Lakshmaiah College of Engineering, Green Fields, Vaddeswaram (India). Production of alkaline amylase by Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran and rice bran were examined. The appropriate Incubation time, Temperature, pH, Inoculum size, Moisture content, Carbon source, Nitrogen source, Substrate concentration, Surfactant and Mutagenic effects were determined. Maximum yield of enzyme was achieved by employing wheat bran as substrate ((94±2) U/g) at 72hrs with 1:1.5 moisture content and pH-8. Inoculum size was found to be 30% for wheat bran. The increased growth of bacterium of mutagenic activity is seen with acrylamide solution. https://www.biotech-asia.org/vol7no2/production-of-alpha-amylase-using-bacillus-subtilis-ncim-no-2724-under-solid-state-fermentation/ Enzymes; alfa-amylase; wheat bran; rice bran