eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 7 2 975 979 9641 article K. Ravi Shankar 1 Sanjoy Das 1 Pavani Bujala 1 Sri Sai Aditya Institute of Pharmaceutical Sciences and Research, A.D.B. Road, Surampalem, Peddapuram - 533 437, East Godavari District (India). The study was carried out to evaluate the qualitative analysis of phytochemicals and in vitro antibacterial activity of Dregea volubilis (L. f.) Benth. ex Hook. f. [Syn. Wattakaka volubilis (L. f.) Stapf., Marsdenia volubilis (L. f.) Cooke and Schollia volubilis (L. f.) Jacq. ex Steud.] (Family: Asclepiadaceae) leaf from its ethanol (continuous hot percolation by ethanol in soxlet apparatus) and aqueous (cold maceration in distilled water for 72 hours) extract. Dried ethanol extract was subjected to preliminary phytochemical screening for the presence of different phytoconstituents and was found to have saponins, coumarins and phytosterols. Ethanol and aqueous leaf extract (ELE and ALE respectively) were subjected to antibacterial screening by disc diffusion method against three gram positive and six gram negative bacteria. ELE was found to be active whereas ALE to be inactive. https://www.biotech-asia.org/vol7no2/phytochemical-screening-and-in-vitro-antibacterial-activity-of-ethanol-and-aqueous-extracts-of-dregea-volubilis-leaves/ ALE; antibiotic; antibacterial; Disc diffusion; Dregea volubilis; ELE; Marsdenia volubilis