eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-04 7 2 635 646 8987 article Hanan Al-youssef 1 Wafaa H.B. Hassan 1,2 Department of Pharmacognosy, Faculty of Pharmacy, King Saud University, Riyadh (Saudi Arabia). Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig (Egypt). In this study we aimed to isolate and characterize the different chemical constituents of the aerial parts of Carissa edulis in addition to investigate the biological activities including anti-inflammatory, diuretics and the effect on cardiovascular system. Chemical investigation of the aerial parts of Carissa edulis afforded eleven compounds, chlorogenic acid -1- ethyl ether-1-methyl ester (1), caffeic acid methyl ester (2), kaempferol (3), quercetin- 3-O-glucoside-7, 3‘, 4‘ trimethyl ether (4), rutin (5), pinitol (6), b-amyrin (7), lupeol (8), stigmasterol glucoside (9), b –sitosterol (10) and b –sitosterol glucoside (11). The structures of compounds 1-6 were unambiguously established by using UV, 1D and 2D NMR spectroscopy and MS spectrometry. This is the first report of isolation of compound 1 from nature. The biological study of the different extracts showed that the butanol extract exerted higher anti-inflammatory effect than ethyl acetate and aqueous extracts. Also the chloroform extract showed the highest diuretic effect. On the other hand, the aqueous extract exerted a significant decrease in the arterial blood pressure at a dose of 200 mg/kg while the petroleum ether extract produced the highest decrease in heart rate at the same dose. https://www.biotech-asia.org/vol7no2/phytochemical-and-biological-studies-of-the-aerial-parts-of-carissa-edulis-growing-in-saudi-arabia/ Carissa edulis; chlorogenic acid derivative; flavonoids; phenolic compounds