eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 7 2 1003 1006 9787 article S. Meenakumari 1 S. Ahmed John 2 Department of Biotechnology, N.M.S.SVN College (Autonomous), Madurai - 625 019 (India). Post Graduate and Research Department of Botany Jamal Mohammed College, Tiruchhirappalli - 620 020 (India). Methylobacterium, a group of organism which oxidizes methane and C1 carbon compounds. Some of these organism forms pink color colonies when they are cultivated in selective media, called Ammonium Mineral Salt Medium. The present investigation is mainly focused to optimize their growth factors, like PH, Temperature, Media modulation and Substrate optimization and their inhibitors of growth. Because Methylobacterium have fertilizer value and they also act as potential Bioinoculant for improving crop production. https://www.biotech-asia.org/vol7no2/optimization-of-media-growth-factors-and-substrate-for-methylobacterium/ Methylotrophs; Methane; C1 Carbon compounds; Bioinoculant; Inhibitors