eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-05 7 2 813 818 9294 article Abhishek Mule 1 R.C. Patil 1 Ujwala Jadhav 2 V.I. Katchi 3 Asha J. Rao 3 S.K. Gaikwad 4 Department of Microbiology, Bhavan’s College, Andheri (W), Mumbai - 400 058 (India). Department of Life Science, University of Mumbai, Santa Cruz (E), Mumbai - 400 098 (India). Department of Zoology, Bhavan’s College, Andheri (W), Mumbai - 400 058 (India). Department Of Cell and Molecular Biology, Rajiv Gandhi Institute of IT and Biotechnology Bharati Vidyapeeth University (India). Oxytetracyclin production by Streptomyces varsovances was done using sweet potato mash medium. Important process parameters like optimum pH ,temperature, tip speed and Kla value for the antibiotic production was determined. Extraction of oxytetracyclin was carried out using boron tetrafluoride, extracted antibiotic was estimated using bioassay and HPLC. New production method was effective and gave 25% more yield as compared to traditional methods, extraction method is promising for large scale production. https://www.biotech-asia.org/vol7no2/large-scale-production-of-oxytetracyclin-using-streptomyces-varsovances/ Oxytetracyclin; pH; temperature; tip speed; boron tetrafluoride