eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-07 7 2 1035 1037 9810 article S. Meenakumari 1 S. Ahmed John 2 1Department of Biotechnology, N.M.S.SVN College (Autonomous), Madurai - 625 019 (India). 2Post Graduate and Research Department of Botany Jamal Mohammed College, Tiruchhirappalli - 620 020 (India). Methylotrophs a group of organism, have the ability to oxidise C1 carbon compounds. This organism promotes the plant growth by producing plant growth hormones. So this organism can be used as bioinoculant to improve crop production. Mass production of Methylobacterium has some constraints. To overcome the problems existing during the mass production of Methylobacterium a new media is formulated with the contents of Methanol, Peptone, and Cycloheximide to prevent the fungal and bacterial contamination. These media favors the growth of effectively since it is a C1 utilizer. https://www.biotech-asia.org/vol7no2/formulation-of-new-media-for-the-mass-production-of-methylotrophs/ C1 Carbon compounds; methylotrophs; methylobacterium; methanol; cycloheximide