eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-03 7 2 603 606 8881 article N. Adib 1 M. Shekarchi 1 A. Dabirsiaghi 2 H. Hajimehdipoor 1 H. Rastegar 1 B. Akbari- Adergani 1 Food and Drug Lab Research Center, Ministry of Health, Tehran (Iran). Department of Pharmaceutics, School of Pharmacy, Azad University of Medicinal Sciences, Tehran (Iran). A simple, rapid and sensitive isocratic reversed-phase high performance liquid chromatography method for the determination of repaglinide in human plasma has been developed. After protein precipitation with acetonitrile chromatographic analysis of repaglinide in plasma was achieved on a μ-bondapack C18 column using acetonitrile- methanol- potassium dihydrogen phosphate 0.01 M (51: 11: 38) mixture, pH 2.5, as mobile phase. The flow rate was set at 1.5 ml/min and the emission and excitation were 348 and 244 respectively. The lower limit of detection was 1 ng/ml and lower limit of quantization was 5ng/ml. The intra and inter-day precisions (CV %) of the quality control samples were 1.43- 3.29 and 1.14- 4.11% respectively. The recovery of method was %98.3 ± 1.08. The method was applied to a bioequivalence study in human. https://www.biotech-asia.org/vol7no2/a-new-hplc-method-for-determination-of-repaglinide-in-human-plasma-and-its-application-in-bioequivalence-studies/ Repaglinide; Human plasma; HPLC; Bioequivalence