eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 7 1 327 332 9574 article V. Sridevi 1 M.V.V. Chandana Lakshmi 1 A.V.N. Swamy 2 M. Narasimha Rao 3 S.V.Naidu 3 Centre for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam - 03 (India). Department of Chemical Engineering, College of Engineering, J.N.T.U., Anantapur (India). Al-Ameer College of Engineering and Information Technology, College of Engineering, Gudilova, Anadapuram, Visakhapatnam (India). Phenol biodegradation by Pseudomonas fluorescence (NCIM 2100) was performed in batch system. Experiments were carried out as a function of inoculum size (1-10 % v/v), p H (5-9) and temperature (27-32°C). Optimization of these three process parameters for phenol biodegradation was studied. Optimum inoculum size, p H and temperature were determined as 5% v/v, 7 and 30°C respectively. At these optimum conditions, the degradation capability of P. fluorescence was carried out at phenol levels (100- 750 mg/l). The maximum phenol degradation of P. fluorescence was 480 mg/l. https://www.biotech-asia.org/vol7no1/optimization-of-process-variables-for-the-microbial-degradation-of-phenol-by-pseudomonas-fluorescence-ncim-2100/ Biodegradation; phenol; Pseudomonas fluorescence; inoculum size; pH; temperature.