eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-06 7 1 445 448 9740 article K.Vanitha Prakash 1 M. Padmalatha 2 Eranna Dopadally 3 SSJ College of Pharmacy, V.N. Pally, Gandipet, Hyderabad (India). Vijaya College of Pharmacy, Munganoor, Hayathnagar, Hyderabad (India). Sultan-Ul-Uloom College of Pharmacy, Banjara Hills, Hyderabad (India). Two simple, economical, precise, reliable and reproducible visible spectrophotometric methods (A and B) have been developed for the estimation of Tenofovir (TNF) in bulk as well as in Tablet formulations. The developed methods A and B are based on the formation of chloroform extractable complex of Tenofovir with Bromocresol green (Method A) and Erichrome (Method B), which shows absorbance maxima at 420 nm and 510 nm respectively. The absorbance-concentration plot is linear over the range of 25-250 mcg/ml for Method A, and 25-200 mcg/ml for Method B. The different experimental parameters affecting the development and stability were studied carefully and optimized. Results of analysis for all the methods were validated statistically and by recovery studies. https://www.biotech-asia.org/vol7no1/extractive-spectrophotometric-determination-of-tenofovir/ Tenofovir; Bromocresol green; Erichrome; Ultraviolet-Visible double beam spectrophotometer.