eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-03 6 2 757 760 8939 article C. Arunachalam 1 R. Rajasekaran 2 PG and Research Department of Microbiology, Sri Venkateshwara College of Arts and Science, Peravurani, Thanjavur (Dt), (India) PG and Research Department of Botany and Microbiology, A Veeriya Vandaiyar Memorial Sri pushpam College (Autonomous), Poondi, Thanjavur - 613 503 (India). Pseudomonas aeruginosa is a bacterium responsible for causing life threatening infections like nosocomical infections, life threatening infections in immune compromised persons. The bacterium’s virulence depends on the numerical abundance of cells associated with extracellular factors. The study was taken up to isolate and to understand the biochemical characterization of P. aeruginosa isolated from pus sample (S1), urine samples (S2) Sputum sample (S3) and soil sample (S4). The multiple drug resistance property of P. aeruginosa was clearly demonstrated by Kirby-Bauer’s disc diffusion method. Pseudomonas aeruginosa (S1) isolated from pus sample was selected for pigment studies, since this strain showed good resistance to various antibiotics than others. Pyocyanin (1-hydroxy-N-Methyl phenazine) is a bluish green, chloroform soluble, cytotoxic pigment secreted by the bacterial species of P. aeruginosa. Tech agar was used for the production of pyocyanin pigment and the pigmented broth tube was centrifuged and the pigment was extracted using choloroform. The extracted pigment showed antibacterial activity against E.coli, Proteus vulgaris and Staphylococcus aureus. https://www.biotech-asia.org/vol6no2/studies-on-antibiotic-resistance-of-pseudomonas-aeruginosa-isolated-from-various-sample-with-special-reference-to-the-antibacterial-activity-of-its-pyocyanin-pigment/ Clinical samples; Pseudomonas aeruginosa; Pyocyanin; and Antibacterial activity