eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-05-04 6 2 807 810 9000 article In Vitro Flowering and Regeneration of Centella Asiatica N. Sarada Mani 1 Rohini Kiran Kunta 1,2 Varahala rao Vadlapudi 1,2 Department of Botany, Andhra University, Vishakhapatnam (India). Dr. Lankapalli Bullyya Post Graduate College, Visakhapatnam (India). In vitro plant regeneration was achieved from explants of nodal segments on MS medium containing different concentrations and combinations of auxins and cytokinins. In vitro flowering was noticed from nodal explants cultured on MS medium supplemented with BAP 2 mg/L + NAA 1mg/L. Multiple shoot induction was observed on MS medium supplemented with BAP + NAA (0.5 - 4 mg/L). The regenerated shoots were transferred onto MS Medium fortified with IBA for root induction. The regenerated plantlets have been successfully established in soil and they were shifted to field condition. https://www.biotech-asia.org/vol6no2/in-vitro-flowering-and-regeneration-of-centella-asiatica/

Plant regeneration; auxins; Multiple shoot induction; MS Medium