eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-04-15 6 1 209 214 8107 article K. Perinbam 1 S. Kanimozhi 2 Keerthigha bharathy 2 V. Ramnath 3 Ellite Bio Science Foundation, Chennai - 1 (India). Asan Memorial College of Arts and Science, Jaladampet, Chennai - 600 100 (India). St. Joseph’s College, Cuddalore (India). The objective of this study is to produce tannase using pure culture Aspergillus foetidus MTCC 3557, Rhizopus oryzae MTCC 1987 and co-culture (Aspergillus foetidus + Rhizopus oryzae) through solid-state fermentation. Using tamarind seed powder as substrate. To compare the yield of tannase by pure and co-culture, to enhance the yield of tannase by using carbon (glucose and tannic acid) and nitrogen sources (ammonium nitrate and sodium nitrate). Results revealed that co-culture system yielded maximum tannase production under desired incubation period of three days. Tannic acid increased the production of tannase when compared to glucose, ammonium nitrate showed minor increase in the production of tannase and the enzyme tannase capable to degrade the tannin by catalyzing the hydrolysis of ester bonds in hydrolysable tannins. https://www.biotech-asia.org/vol6no1/production-of-enzyme-tannase-by-solid-state-fermentation-using-tamarind-seed-powder/ Tannase; carbon source; tamarind seed poweder; fungi; debittering