eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-04-21 6 1 393 395 8458 article A. R. Rout 1 S. C. Kheti 1 T. Mohapatra 1 A. Tripathy 2 Mahatma Gandhi College of Pharmaceutical Science, Jaipur, Rajasthan (India). Apex College of Pharmaceutical Science, Jaipur, Rajasthan (India). The concept of protein estimation analysis has highly effective on the study of plant reaction in environmental condition. The determination of quantitative protein analysis of plant will vary according to the environmental factors and nutrient application in form of fertilizer and bio-inoculants. The following study specifies the characterization of plant Benzolive tree (Moringa oleifera) PKM1 variety by estimating protein and polyphenol from leaf sample with standardization of Bovine Serum Albumin (BSA) and Gallic acid respectively. https://www.biotech-asia.org/vol6no1/leaf-protein-estimation-of-benzolive-tree-by-using-different-bioinoc4ulants-and-fertilizers/ Polyphenol; protein; BSA and gallic acid