eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-04-16 6 1 293 296 8189 article Mallikarjun Malipatil 1 H. R. Chitme 2 Ramesh Chandra 3 H. Ramesh 4 Raghuveer Irrchaya 5 Karnataka College of Pharmacy, Manhalli Road, Bidar - 585 403 (India). Department of Pharmacy Azaiba, Muscat Sultanate of Oman (Oman). Founder director, Dr.B.R.Ambedkar Center for Biomedical Research Delhi University, Delhi (India). R.R.K. College of Pharmacy Nauabad Bidar (India). Head and Director Institute of Pharmacy Bundelkhand University Jhansi (India). Carissa carandas Linn (Family: Apocynaceae) commonly known as Karaunda, is a large evergreen shrub with a short stem, glabrous shrub found almost through out India. All the parts of the Carissa carandas linn were reputed in indigenous medicine for various kinds of diseases and disorders. The objective of the work is to study the chemical constituents of benzene extract of root of Carissa carandas Linn. The fresh root of the plant is used internally as anthelmentics, antipyretic, stomachic and antiscorbutic. Phytochemical investigation conducted on root revealed the presence of carbohydrates, alkaloids, flavanoids, saponins, steroids and fatty acids. The aromatic amide was detected in benzene extract and methanol extract of the root. The extracts were carried out by soxhlet extractor and the yield of benzene extract was 2.5%.The TLC study of methanolic extract showed the presence of 4 spots. The three spots were identified as aromatic amides. The present study deals with isolation characterization of aromatic amide (M3) isolated from methanolic extract of root of Carissa carandas linn. The four compounds were separated by column chromatography using benzene: ethyl acetate: chloroform (4:2:1). The TLC study of separated compounds with Rf value 0.85 (M1), 0.73 (M2), 0.68 (M3) and 0.65 (M4). From UV, IR, 1HNMR and LC-Mass spectral analysis of the compound M3 .It could be characterized as aromatic amide. https://www.biotech-asia.org/vol6no1/isolation-and-characterization-of-aromatic-amide-from-methanolic-extrat-of-root-ofcarissa-carandas-linn/ Carissa carandas; methanolic extract; aromatic amide; TLC; column chromatography