eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-04-04 5 2 763 766 7557 article Aara Rifat 1 S.A. Hajam 1 K.I. Andrabi 1 Department of Biochemistry, University of Kashmir, Kashmir - 190 006 (India). A heat stable protein (hsp) has been purified to homogeneity from sheep liver extract by heating to 95 O C followed by ion-exchange chromatography on DEAE-cellulose (DE-52). The purified protein showed a single band when examined by gel electrophoresis. The molecular weight determined by SDSpage was found to be approximately 70KDa. The presence of this protein was also demonstrated in whole cell homogenate, heat stable and nuclear fraction. https://www.biotech-asia.org/vol5no2/purification-and-identification-of-70-kda-heat-stable-protein/ Hsp; SDS-page