eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-14 5 2 651 656 7214 article Z. Bouchikhi Tani 1 M. A. Khelil 1 F. Hassani 2 Laboratory of Valorisation of the Actions of the Man for the Environmental Protection and Application in Public Health - Tlemcen (Algeria). Laboratory of Ecology and Management of the Natural Ecosystems - Tlemcen (Algeria). In the present study we tested the insecticidal capacity of the essential oils extracted from Rosmarinus officinalis, on two devastating beetles, Acanthoscelides obtectus and Tineola bisselliella (coleopters and lepidopteres). Bioassays on A. obtectus were realized under the conditions of laboratory (temperature and relative humidity respectively maintained to 27°C and 75%), and on T. bisselliella has a temperature of 25°C and relative humidity of 75%. Essential oils were extracted from the leaves of R. officinalis by hydrodistillation (yield of 0,6%). The doses used out of essential oils on A. obtectus are 1 to 5 μ l/30g of seeds, and 1 to 5 μ L on T. besselliella. The results show that essential oils tested are very toxic on adulate A. obtectus with LD50 = seed 0,59μL/30g after 48h of exposure, as it appears that the mite T. bisselliella is also very sensitive with respect to essential oils with LD50 = 1,28 μ L. https://www.biotech-asia.org/vol5no2/fight-against-the-bruche-bean-acanthoscelides-obtectus-coleoptera-bruchidae-and-the-mite-tineola-bisselliella-lepidoptera-tineidae-by-the-essential-oils-extracted-from-rosmarinus-officinalis/ canthoscelides obtectus; Tineola bisselliella; Rosmarinus officinalis; essential oils