eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-12 5 2 593 600 7175 article Nizam Kassim Sahidatul 1 Kalavani Nadarajah 1 Ismail Bin Ahmad 1 School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43650 Bangi, Selangor (Malaysia) The recombinant plasmid, pSNC3301 carrying the coat protein gene of cucumber mosaic virus (CpCMV) was used in the Agrobacterium-mediated transformation of chili (Capsicum annuum L.). The transformation protocol required 2 days co-cultivation, 2 days pre-selection in non-BASTATM herbicide containing medium, 3-4 weeks selection in BASTATM herbicide containing medium, 20-25 weeks for culturing of adventitious shoot in regeneration medium and finally 1 week in rooting medium. PCR analysis detected the presence of CpCMV gene with transformation efficiency of 1.5% (7/467) for hypocotyl and 0.7% (4/536) for shoot tip. Histochemical GUS assay of hypocotyls, showed only nine from 22 PCR positive transformants showed GUS expression. For shoot tip, three of 13 PCR-positive transformants expressed GUS. The transformation efficiency of coat protein using pSNC3301 was 1.5% (7/467) for hypocotyls and 0.7% (4/536) for shoot tip.re https://www.biotech-asia.org/vol5no2/feasibility-decrease-use-of-fertilizer-on-greenhouse-cucumber-production-in-soilless-culture-2/ Capsicum annuum; shoot tip; hypocotyls; transformation; Agrobacterium Coat protein; CMV