eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-04-02 5 2 719 726 7503 article Ripudaman Singh 1 R. Irchhaiya 1 Manjulata 1 R. K. Arya 1 A. K. Gupta 1 Institute of Pharmacy, Bundelkhand University, Jhansi - 284128 (India). A series of mucoadhesive microspheres of ofloxacin have been successfully prepared, using chitosan as a polymer cross-linked with glutaraldehyde as the shell. The morphology was characterized by scanning electron microscopy (SEM). The swelling and releasing behaviors of the microspheres at pH 1.2 were investigated. The results revealed that microsphere exhibited very smooth and spherical surface and electrostatic interaction existed between cross-linked chitosan and ofloxacin. Ofloxacin bounded in the microspheres was slowly released in 0.1N HCl (pH 1.2) because the degree of swelling of the microsphere was very low. The release mechanism of the mucoadhesive microsphere was through the swollen microspheres and to be controlled by the cross-linking shell density of the microspheres. The higher the cross-linking density of, the lower is the swelling ability of the chitosan microsphere due to the slower relaxation of polymer chain, which then results in decreased drug release rate. https://www.biotech-asia.org/vol5no2/development-and-characterization-of-mucoadhesive-microspheres-of-ofloxacin/ Mucoadhesive; microspheres; ofloxacin