eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-01 5 1 337 342 6843 article M. Vijey Aanandhi 1 Shiny George 1 V. Vaidhayalingam 2 Department of Pharmaceutical Chemistry, Vel’s College of Pharmacy, Chennai - 600 117 (India) Department of Pharmaceutical Chemistry, Madras Medical College, Chennai - 600 003 (India) Some novel Mannich base isatin derivatives were synthesized by reacting 1-(5-methoxy-2-oxoindolin-3-ylidene)-4-(substituted pyridin-2-yl)thiosemicarbazide with formaldehyde and several secondary amines. Their chemical structure was elucidated by means of spectral (FT-IR, 1H NMR and mass) analysis. Investigation of analgesic activity of synthesized compounds was done by acetic acid induced writhing reflex method using diclofenac sodium as standard drug. The synthesized compounds showed significant analgesic activity. https://www.biotech-asia.org/vol5no1/synthesis-and-analgesic-activity-of-1-1-substituted/ Isatin; Schiff base; Mannich base; analgesic activity