eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-09 5 1 6989 article Spectrophotometric Estimation of Tenofovir in Pharmaceutical Formulations Appala Raju 1 J. Venkateswara Rao 1 K. Vanitha Prakash 2 K. Mukkanti 3 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road 3, Banjara Hills, Hyderabad - 500 034 (India) Centre For Environment IST Building, JNTU, Kukatpally, Hyderabad - 500 072 (India) Three simple, accurate, rapid and sensitive methods (A, B and C) have been developed for the estimation of Tenofovir in its pharmaceutical dosage form. The Method A is based on the formation of orange red colored chromogen, due to reaction of Tenofovir with p-Dimethyl amino cinnamaldehyde (PDAC) reagent in methanol, which exhibits l max at 530 nm. Method B is based on the reaction of Tenofovir with 4-aminophenazone (4-AP) in the presence of Sodium periodate to form a intense violet colored chromogen, which shows maximum absorbance at 545 nm. The Method C is based on the formation of blood red colored chromogen with Ferric Chloride and 1,10-phenanthroline which shows absorption maximum at 512 nm. The absorbance-concentration plot is linear over the range of 1-7 mcg/ml for Method A, 5-50 mcg/ml for Method B and 1-15 mcg/ml for Method C. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are economical and sensitive for the estimation of Tenofovir in bulk drug and in its formulations. https://www.biotech-asia.org/vol5no1/spectrophotometric-estimation-of-tenofovir-in-pharmaceutical-formulations/

UV-Visible Spectrophotometry; Tenofovir; Ferric chloride; methanol; Sodium periodate