eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-01 5 1 359 362 6855 article Ajay Kumar Das 1 Bhaskar C. Harinath 2 Department of Biochemistry, Agartala Government Medical College, Kunjaban, Agartala - 799 006 (India) Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha - 442 102 (India) Immuno screening was carried out by ELISA for the samples from the patients admitted in Kastruba Hospital, Sevagram. Blood samples of patients of pulmonary and extra pulmonary tuberculosis were examined for presence of tuberculosis specific antibody, antigen and combine sensitivity was also tested. Antigen preparation namely M.tbH37Ra ES-31 and 41 were used for the detection of tubercular IgG antibody whereas affinity purified anti ES-31 antibody was used for tubercular circulating free and immune complexed (CIC) antigen. ES-31 showed more sensitivity in pulmonary, lymphnode, CNS, genito uninary TB and ES-41 showed more in abdominal, osteo articular TB, percentage positivity in combined Ab/ Ag/ CIC-Ag sensitivity was found more. https://www.biotech-asia.org/vol5no1/comparative-analysis-of-es-31-and-es-41-antigens-in-the-detection-of-tubercular-igg-antibodies-and-study/ ES-31; ES- 41; Pulmonary; Extra-pulmonary; Tuberculosis