eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-02-03 4 2 595 602 5997 article V. Vijayalatha 1 B. Singh 1 Tarun Chandra Bora 2 Rakha Hari Das 1 Hemant K. Gautam 1 Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi - 110 007 (India) Regional Research Laboratory, Jorhat, Assam - 785 006 (India) A thermophilic bacterium Brevibacillus borstelensis strain RH 102 was isolated from the municipal garbage dumping site through plate exposure method. The cell free supernatants (CFS) of this strain were screened for antimicrobial activity by agar diffusion assays against gram positive, gram negative and fungal pathogens. Methanolic extracts of CFS showed antimicrobial activity against the gram positive bacteria. The 16S rRNA gene sequence of this strain showed 99% nucleotide sequence homology with that of Brevibacillus borstelensis R-16402. The antimicrobial activity of the methanolic extract of CFS of RH 102 is abolished by protease. The protease sensitivity of the antimicrobial(s) produced by this Brevibacillus strain indicates it to be peptidyl in nature. Present study reports the production of antimicrobial compounds for the first time by Brevibacillus borstelensis. Selective spectrum activity of this metabolite(s) can have potential importance against emerging resistant gram positive pathogens. https://www.biotech-asia.org/vol4no2/selective-antimicrobial-profile-of-a-newly-isolated-thermostable-brevibacillus-borstelensis/ Brevibacillus borstelensis; antibacterial activity; thermophile; 16S rRNA gene sequencing